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An important component of these defenses is the accumulation of pathogenesis-related (PR) proteins ( Sels et al. Upon recognition of general elicitors, plants induce basal defenses that confer resistance to a wide range of pathogens. These results demonstrate the importance of plant chitinase cleavage in fungal virulence. oxysporum lacked chitinase cleavage activity on SlChi1 and SlChi13 and showed reduced virulence on tomato. Double deletion mutants of FoMep1 and FoSep1 of F. Both secreted metalloprotease FoMep1 and serine protease FoSep1 were responsible for this cleavage.
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oxysporum removed the CBD from the N-terminus, shown by mass spectrometry, and significantly reduced the chitinase and antifungal activity of both chitinases. lycopersici, Verticillium dahliae, and Botrytis cinerea were able to cleave the extracellular tomato chitinases SlChi1 and SlChi13.
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Four tomato CBD chitinases were produced in Pichia pastoris and were incubated with secreted proteins isolated from seven fungal tomato pathogens. Here, we investigated the ability of fungal tomato pathogens to cleave chitin-binding domain (CBD)-containing chitinases and its effect on fungal virulence. However, it is not known how fungal pathogens that lack chitin-binding effectors overcome this plant defense barrier. Some fungi are not sensitive to plant chitinases because they secrete chitin-binding effector proteins that protect their cell wall against these enzymes. As part of their defense strategy against fungal pathogens, plants secrete chitinases that degrade chitin, the major structural component of fungal cell walls.